علوم زیستی دریا
Hassan Morovvati; Mahmood Khaksary Mahabady; Mehrzad Mesbah; Mehdi Hadi Jafari
Abstract
Barbus grypus is native fishes in Khuzestan province which its species with a wide distribution in the rivers that considered fisheries research in province. The fish have no stomach, space of intestine is narrow to store of food, for this reason anterior intestine dilated bowel and called intestinal ...
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Barbus grypus is native fishes in Khuzestan province which its species with a wide distribution in the rivers that considered fisheries research in province. The fish have no stomach, space of intestine is narrow to store of food, for this reason anterior intestine dilated bowel and called intestinal bulb. Study of histological structure of intestinal bulb in fishes can help to nutrition and food needs so that we can extend of this species. In this study, 10 adults Barbus grypus of both sexes with average weigh 354.25 ± 60.52 g and average length 36.25 ±4.16 cm was chosen, and after easy drawing, samples were removed from intestinal bulb. The 0.5cm were fixed by Bouin´s fixative soluble and 5-6µ thickness sections were made by paraffin embedding method and were stained by H&E and PAS. The histomorphometrical studies were done using digital Dino-Lite lens and Dino-capture1 software. The results showed that the number of goblet cells in the intestine increased toward the end of the intestinal bulb. The intestinal epithelium height in the anterior part of the intestinal bulb was higher than other parts. Muscular thickness was different in different parts of the intestinal bulb, the middle part of intestinal bulb has thickest muscle between other parts of intestinal bulb. The results of this research indicate that there is some analog histological structure of the intestinal bulb in Barbus grypus to compare of other same family: from wall diameter and intestinal epithelium height and diameter of mucosa.
علوم زیستی دریا
Hassan Morovvati; M Mesbah; MM Shamsi; A Rezae; Kh Rashidi
Abstract
For this purpose, 144 specimens were studied in five healthy groups. The first group was the control group put in dechlorinated tap water and four respectively in the next group were kept with salinity 4ppt, 8ppt, 12ppt, 16ppt in the same condition . In days (1, 3, 7, 14, 21, 28) samples were collected ...
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For this purpose, 144 specimens were studied in five healthy groups. The first group was the control group put in dechlorinated tap water and four respectively in the next group were kept with salinity 4ppt, 8ppt, 12ppt, 16ppt in the same condition . In days (1, 3, 7, 14, 21, 28) samples were collected from skin of head, ventral and lateral lines. Sections with 0.5- 0.6µm thickness were made and stained with PAS and H&E methods. In histometrical survey, epiderm thickness, numbering of club cells and goblet cells in 200 µm of epiderm length in any area, was investigated comparatively. The results showed that in different salinity concentrations, skin of head, ventral and lateral lines were influenced and changes in epiderm thickness, reduce the number of club cells and increase the number of goblet cells were demonstrated . These changes in different concentrations for each parameter , for significantly increased (p